Solid-Phase Metal Chelate Assay for Quantifying Total Protein: Resistance to Chemical Interference
نویسندگان
چکیده
منابع مشابه
Solid-phase metal chelate assay for quantifying total protein: resistance to chemical interference.
Recently, we developed reversible metal chelate stains that are fully compatible with immunoblotting and protein sequencing. Membrane supports are incubated in Ferrozine/ferrous complex followed by ferrocyanide/ferric complex (double-metal chelate [DMC] stain). Proteins are quantified by computerized densitometry. In this study, the metal chelate stains are used for routine protein quantitation...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96215rr01